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Uji aktivitas antibakteri ekstrak etanol daun pegagan (Centella asiatica) terhadap bakteri Streptococcus pneumoniae secara in vitro

Abstract

Background: The gotu kola plant (Centella asiatica) is one of the herbal ingredients used throughout the world. This plant contains triterpenoids and saponins which have broad therapeutic functions. Streptococcus pneumoniae has become a global problem that causeing community acquired pneumonia the most in the world. Our study investigate further the antibacterial activity of ethanol extract of Centella asiatica leaves to inhibit Streptococcus pneumoniae bacteria.

Methods: The Kirby-Bauer method was used to test the antibacterial activity of gotu kola (Centella asiatica) extract in this study. This is done by measuring the diameter of the inhibition zone and its standard deviation.

Results: In the positive control group of Vancomycin 30 µg, the diameter of the inhibition zone was found to be 26.7 mm, 27.15 mm and 28.05 mm. At concentrations of 25%, 50% and 75% of gotu kola leaf (Centella asiatica) ethanol extract, no antibacterial activity was found. Meanwhile, inhibition zone diameters of 8.3 mm, 8.4 mm, and 8.1 mm were found in the ethanol extract of gotu kola leaves (Centella asiatica) with a concentration of 100%.

Conclusion: Based on the results obtained, the concentration of 100% ethanol extract of gotu kola leaves (Centella asiatica) has antimicrobial activity but is unable to inhibit the growth of Streptococcus pneumoniae bacteria.

 

Latar Belakang: Tanaman pegagan (Centella asiatica) merupakan salah satu bahan herbal yang digunakan di seluruh dunia. Tanaman ini memiliki kandungan utama triterpenoid dan saponin yang berfungsi sebagai terapeutiknya yang luas. Streptococcus pneumoniae adalah bakteri yang secara historis menjadi patogen paling umum yang menyebabkan pneumonia komunitas di seluruh dunia. Penelitian mengevaluasi lebih lanjut terkait aktivitas antibakteri ekstrak etanol daun pegagan terhadap bakteri Streptococcus pneumoniae.

Metode: Metode Kirby-Bauer digunakan untuk pengujian aktivitas antibakteri ekstrak pegagan (Centella asiatica) pada penelitian ini. Hal ini dilakukan dengan mengukur diameter zona hambat dan standar deviasinya.

Hasil: Pada kelompok kontrol positif Vancomycin 30 µg, ditemukan diameter zona hambat sebesar 26,7 mm, 27,15 mm, dan 28,05 mm. Pada konsentrasi ekstrak etanol daun pegagan 25%, 50%, dan 75% tidak ditemukan aktivitas antibakteri. Sedangkan, diameter zona hambat sebesar 8,3 mm, 8,4 mm, dan 8,1 mm ditemukan pada ekstrak etanol daun pegagan (Centella asiatica) konsentrasi 100%.

Kesimpulan: Penelitian ini menunjukkan bahwa konsentrasi ekstrak etanol daun pegagan (Centella asiatica) 100% mampu menunjukan aktivitas antimikroba tetapi tidak dapat menghambat pertumbuhan bakteri Streptococcus pneumoniae.

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How to Cite

Purba, R., Hendrayana, M. A. ., Budayanti, N. N. S. ., & Fatmawati, N. N. D. . (2024). Uji aktivitas antibakteri ekstrak etanol daun pegagan (Centella asiatica) terhadap bakteri Streptococcus pneumoniae secara in vitro. Intisari Sains Medis, 15(1), 109–113. https://doi.org/10.15562/ism.v15i1.1943

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Rizky Purba
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Made Agus Hendrayana
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Ni Nyoman Sri Budayanti
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Ni Nengah Dwi Fatmawati
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